Coding
Part:BBa_K2984052:Design
Designed by: Darius Rauch Group: iGEM19_Humboldt_Berlin (2019-10-16)
L1c-PsadIntron-bleRscp-ARS-YFP-Rbcs2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2051
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2051
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2051
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2051
Illegal NgoMIV site found at 1607
Illegal NgoMIV site found at 2528 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Secretion construct for C.reinhardtii designed for transformation in Clamydomnasd and further cloning into Level 2 backbone with MoClo standard.
Source
The construct consist of the five different Level 0 parts PsadIntron (BBa_K2984046), bleRscp (BBa_K2984045), ARS (BBa_K2984000), YFP (BBa_K2984017) and Rbcs2(BBa_K2984018) which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo standard